PCR Enzyme
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Kit Contents
10×Long Taq Buffer
It is supplied with two high-efficiency amplification buffers, i.e. 10×Long Taq Buffer I and Buffer II, which are suitable for the amplification of different templates. Please use Buffer I first, and try Buffer II again when Buffer I cannot perform well.
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Storage
Store at -20℃. -
Storage Buffer
20 mM Tris-HCl (pH8.0)
0.1 mM EDTA
1 mM DTT
100 mM KCl
50% Glycerol
Stabilizers
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Description
Long Taq DNA Polymerase is a Taq DNA polymerase developed by TIANGEN with 5'-3' exonuclease activity and 3'-5' exonuclease activity. It has the characteristics of high amplification efficiency and high fidelity. Equipped with two efficient amplification Buffer, it can adapt to the amplification of different templates. For simple templates, it can amplify up to 40 kb of fragments, and for complex secondary structures (GC rich, etc.) and templates with repetitive sequences, it can amplify up to 15 kb of fragments.
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Activity Definition
1unit (U) Long Taq DNA Polymerase activity is defined as the amount of enzyme required to incorporate 10 nmol deoxynucleotides into acid-insoluble substances at 74°C within 30 min using activated salmon sperm DNA as template/primer.
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Quality Control
The purity of SDS-PAGE is more than 99%; No activity of exogenous nuclease is detected; Single gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week. -
Main Technical Parameters
It has 5'-3' exonuclease activity and 3'-5' exonuclease activity. PCR products can be directly cloned into TA vector. If the cloning efficiency needs to be improved, it is recommended to purify the PCR products first and add with 3'-dA overhang before cloning into TA vector. -
Applications
It is suitable for amplification of long fragments with high fidelity requirements and some complex templates (such as secondary structure, rich GC sequence and repeat sequence, etc.), such as gene mapping, sequencing and molecular genetics research, etc.
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